The Synaptonemal Complex Protein SCP3 Can Form Multistranded, Cross-striated Fibers In Vivo

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The Synaptonemal Complex Protein SCP3 Can Form Multistranded, Cross-striated Fibers In Vivo

The synaptonemal complex protein SCP3 is part of the lateral element of the synaptonemal complex, a meiosis-specific protein structure essential for synapsis of homologous chromosomes. We have investigated the fiber-forming properties of SCP3 to elucidate its role in the synaptonemal complex. By synthesis of SCP3 in cultured somatic cells, it has been shown that SCP3 can self-assemble into thic...

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The meiotic specific synaptonemal complex protein SCP3 is expressed by female and male primordial germ cells of the mouse embryo.

The synaptonemal complex proteins SCP3 and SCP1 are components of the synaptonemal complex, a meiosis-specific protein structure essential for synapsis of homologous chromosomes. Using polyclonal antibodies raised against SCPs of rat testis, we have studied the expression of these proteins in embryonic germ cells of the mouse embryo using immunohistochemistry and immunoblotting. This investigat...

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In vivo analysis of synaptonemal complex formation during yeast meiosis.

During meiotic prophase a synaptonemal complex (SC) forms between each pair of homologous chromosomes and is believed to be involved in regulating recombination. Studies on SCs usually destroy nuclear architecture, making it impossible to examine the relationship of these structures to the rest of the nucleus. In Saccharomyces cerevisiae the meiosis-specific Zip1 protein is found throughout the...

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Voltage Clamp Experiments in Striated Muscle Fibers

1. Membrane currents during step depolarizations were determined by a method in which three electrodes were inserted near the end of a fibre in the frog's sartorius muscle. The theoretical basis and limitations of the method are discussed.2. Measurements of the membrane capacity (C(M)) and resting resistance (R(M)) derived from the current during a step change in membrane potential are consiste...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1998

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.142.2.331